Better Gel Resolution and Longer cDNAs Increase the Precision of Differential Display
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چکیده
منابع مشابه
Better gel resolution and longer cDNAs increase the precision of differential display.
become a widely used method for the rapid identification of differentially expressed genes in a variety of eukaryotic systems (4). In the past three years, technical modifications have reduced the redundancy of anchored primers, decreased the number of reverse transcription reactions needed, increased reproducibility, reduced the incidence of false positives and increased the overall efficiency...
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15 صفحه اولIdentification and verification of differential display cDNAs using gene-specific primers and hybridization arrays.
An accurate and streamlined approach to differential display (DD) band identification and verification is described. To minimize false positives, the strategy avoids the use of impure Northern blot probes obtained from PCR-amplified DD bands. To increase throughput, the cloning of DD bands is replaced by a gene-specific primer approach, and hybridization arrays are used in place of Northern blo...
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Differential display, first described by Liang and Pardee (4), has become a powerful screening tool for the identification of cellor stage-specific gene expression. The technique relies on efficient reverse transcription of mRNA and subsequent amplification of this cDNA with as many random primer combinations as possible. RT-PCR products are separated on denaturing sequencing gels, visualized t...
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Differential display polymerase chain reaction is technically challenging at a number of steps, including reliable reamplification of differentially expressed sequences after they are isolated from an acrylamide gel. As the only source of the sequence found to be of interest is the gel from which it was identified as being differentially expressed, failed attempts at reamplification can be part...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96205pf02